Purification

At Videti, a trusted Contract research and development organization (CRDO) in hyderabad, purification services are essential for producing high-quality pharmaceuticals, chemicals, and biotech products. Techniques such as HPLC, flash chromatography, crystallization, and solid-phase extraction are employed to isolate and purify target compounds from complex mixtures, ensuring the highest levels of purity and consistency.

In pharma, purification is critical for APIs, excipients, biologics, and vaccines, where even trace impurities impact safety and efficacy. Growing demand for sustainable methods has driven adoption of greener techniques like membrane filtration and supercritical fluid chromatography. These services are vital across industries for delivering safe, effective, and compliant products..

● Flash Chromatography

Flash chromatography is a fast and efficient technique for purifying organic compounds, using a pressurized mobile phase and a silica gel column to separate components based on their affinities. Known for its speed and simplicity, it’s widely used in research and industry for purifying small to medium-scale compounds.

 

● Semi and Preparative HPLC

Preparative HPLC is a high-precision technique used to isolate and purify larger quantities of compounds, typically from milligrams to grams. Unlike analytical HPLC, it’s optimized for bulk purification and widely used in pharmaceuticals, biotechnology, and chemistry for APIs, peptides, and synthetic intermediates.

 

● Super Critical Fluid Chromatography (SFC)

Supercritical Fluid Chromatography (SFC) uses supercritical carbon dioxide CO₂ , as the mobile phase to separate the compounds.Combining gas-like diffusivity with liquid-like solubility, SFC excels at separating non-polar, thermally sensitive, and high-molecular-weight compounds with high resolution and speed.

SFC operates under high pressure with a solid stationary phase and often uses modifiers to tune polarity. It is scalable and environmentally friendly, reducing hazardous waste by replacing organic solvents. Widely used in pharmaceuticals and biotechnology, SFC is especially valuable for chiral separations and sustainable, high-purity isolations.